The aim of this study was to standardize the extractive solution of Syzygium cumini. The extractive solution was selected through a full factorial design, considering the extraction method (maceration-5-days, turbo extraction, percolation), extractor solvent (ethanol 50, 70, 96%) and plant material particle size 0.302 mm into a plant:solvent ratio (1:10), using the dried residue and antimicrobial activity determination as evaluation criteria. The tests were performed in triplicate. It appeared that the percolation is the best procedure extractive, and ethanol mixture: water (50:50) the best solvent. ANOVA analysis showed the importance of these parameters.

Considerando-se a qualidade dos fitoterápicos, é importante salientar que a preocupação com esta questão inclui rigoroso acompanhamento das diferentes etapas do desenvolvimento e produção destes produtos, desde a coleta do vegetal até a disponibilidade do produto final. Neste trabalho, foram realizados o controle da qualidade, o potencial antioxidante como também ensaios biológicos in vitro do fruto da goiabeira (Psidium guajava L.), para o desenvolvimento de uma formulação fitocosmética. Os resultados obtidos mostraram que o fruto apresenta taninos e flavonóides, bem como atividades antioxidante e antimicrobiana. A análise microbiológica não apresentou crescimento de patógenos na formulação desenvolvida entre os outros testes realizados. Destaca-se a importância do estabelecimento do controle da qualidade para as plantas, a fim de que sejam utilizadas para o desenvolvimento de uma formulação fitocosmética segura, eficaz e com qualidade.

Considering the quality of the phytotherapic products, it is important to point out that the concern with this question includes rigorous accompaniment of the different stages of the development and production of these products, since the collection of the vegetable until the availability of the end product. In this work the quality control, the antioxidant potential were performed as also biological assays in vitro of guava fruit (P. guajava L.), for the development of a phytocosmetic formulation. The results had shown that the fruit presents tannins and flavonoids, as well as antioxidant and antimicrobial activities. The microbiological analysis did not present growth of pathogens. The importance of the establishment of the quality control for the plants is relevant, so that they are used for the development of a phytocosmetic formulation considered safe, efficient and with quality.

We evaluated the potential antitubercular activity of triterpenes obtained from leaves and bark of Byrsonima crassa. From chloroform extracts of the leaves, by bioassay-guided fractionation, we obtained mixtures of known triterpenes: α-amyrin, β-amyrin and their acetates, lupeol, oleanolic acid, ursolic acid and α-amyrinone. Tested against Mycobacterium tuberculosis, the triterpenes exhibited minimum inhibitory concentrations (MICs) of 31.25 - 312.25 µg/mL. β-amyrin and friedelin, isolated from the chloroform extract of bark, showed MICs of 312.25 and 125 µg/mL respectively. This is the first report of the identification and determination of the activity of B. crassa triterpenes against M. tuberculosis.

Estudou-se a absorção de magnésio por raízes destacadas de quatro cultivares de tomateiro (Lycopersicon esculentum Mill) em concentrações externas crescentes do elemento, entre 0,06 e 0,41 mmol.L-1. As velocidades máximas de absorção estimadas (Vmáx) foram 4,50; 4,31; 3,93 e 3,42 <FONT FACE="Symbol">m</font>mol.g-1. 40 min-1 para as cultivares Kadá, Yokota, Miguel Pereira e Ângela Hiper, respectivamente, e os valores de Km estimados foram 0,43; 0,35; 0,23 e 0,22 <FONT FACE="Symbol">m</font>mol.L-1, nessa mesma ordem. Com base nas estimativas de Km concluiu-se que os cultivares Ângela Hiper e Miguel Pereira apresentaram carregadores com maior afinidade pelo íon Mg2+ que os cultivares Yokota e Kadá. As Vmáx indicam, entretanto, que tais cultivares provavelmente apresentaram menor número de carregadores desse íon operando na raiz.

The absorption of magnesium by excised roots of tomato plants (Lycopersicon esculentum Mill) was studied in four cultivars (Angela Hiper, Miguel Pereira, Kadá e Yokota). External Mg concentrations were between 0.06 and 0.41 mmol.L-1. Estimated maximum uptakes (Vmax) were between 4.50 and 3.42 mmol.g-1 per 40 minutes, while estimated Km values were between 0.43 and 0,22 mmol.L-1 per 40 minutes. Based on Km estimated values it can be concluded that Ângela Hiper and Miguel Pereira cultivars have higher affinity with the Mg2+ ion carrier than Yokota and Kada cultivars. Maximum Mg uptakes were observed for Ângela Hiper and Miguel Pereira cultivars, that have a lower number of carrier molecules in the roots.